Gu et. al. (Cancer Res 62: 3428-3437, 2002)

Published Data In Robert Clarke Laboratory

Gu et al. Cancer Res 62:3428-3437, 2002

Overview

This site has links to selected data and the online reprint of:

"Association of Interferon Regulatory Factor-1, Nucleophosmin, Nuclear Factor Kappa - B, and Cyclic AMP Response Element Binding with Acquired Resistance to Faslodex (ICI 182,780)." Gu et al., Cancer Res 62: 3428-3437, 2002.

Overview

Both serial analysis of gene expression (SAGE) and gene expression microarrays were used to explore the transcriptomes of antiestrogen responsive and resistant human breast cancer cells. Structure of the gene microarray expression data was visualized using our algorithm that derives the first three principal components, fitted to the antiestrogen resistant and responsive gene expression data, from Fisher's information matrix. Differential regulation and functional studies of the basal expression and endocrine regulation of transcriptional activation of several candidate genes is reported. The results implicate a new signal transduction pathway in which antiestrogen resistant cells upregulate some estrogen responsive genes and concurrently losing the ability of antiestrogens to regulate expression of these genes while also by bypassing specific growth inhibitory signals induced by antagonist-occupied estrogen receptors.

Links to Paper and Data

Link to Paper (at Cancer Research Web Site)
SAGE Data (adapted from Table 2 in paper)
Gene expression microarray data (adapted from Table 3 in paper)
SAGE Database - searchable